Open AccessApplication of Amplicon-Based Targeted NGS Technology for Diagnosis of Drug-Resistant Tuberculosis Using FFPE Specimens
Author(s) -
Jing Song,
Wei Du,
Zichen Liu,
Jialu Che,
Kun Li,
Nanying Che
Publication year - 2022
Publication title -
microbiology spectrum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.502
H-Index - 51
ISSN - 2165-0497
DOI - 10.1128/spectrum.01358-21
Subject(s) - amplicon , tuberculosis , computational biology , drug resistant tuberculosis , drug , drug resistance , mycobacterium tuberculosis , biology , medicine , genetics , polymerase chain reaction , pharmacology , pathology , gene
Amplicon-based targeted NGS technology focuses on a set of gene mutations of known or suspected associations with drug susceptibility inMycobacterium tuberculosis (MTB). This method offers many benefits, such as low sequencing cost, easy customization, high throughput, shorter testing time and not culture dependent.