Library Preparation and Sequencing Platform Introduce Bias in Metagenomic-Based Characterizations of Microbiomes | Zendy

Casper Sahl Poulsen | Zendy; Claus Thorn Ekstrøm | Zendy; Frank M. Aarestrup | Zendy; Sünje Johanna Pamp | Zendy

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Library Preparation and Sequencing Platform Introduce Bias in Metagenomic-Based Characterizations of Microbiomes

Author(s) -

Casper Sahl Poulsen,

Claus Thorn Ekstrøm,

Frank M. Aarestrup,

Sünje Johanna Pamp

Publication year - 2022

Publication title -

microbiology spectrum

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.502

H-Index - 51

ISSN - 2165-0497

DOI - 10.1128/spectrum.00090-22

Subject(s) - metagenomics , microbiome , computational biology , biology , evolutionary biology , computer science , data science , genetics , gene

Previous research has reported effects of sample storage conditions and DNA isolation procedures on metagenomics-based microbiome composition; however, the effect of library preparation and DNA sequencing in metagenomics has not been thoroughly assessed. Here, we provide evidence that library preparation and sequencing platform introduce systematic biases in the metagenomic-based characterization of microbial communities.

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