Open AccessLibrary Preparation and Sequencing Platform Introduce Bias in Metagenomic-Based Characterizations of Microbiomes
Author(s) -
Casper Sahl Poulsen,
Claus Thorn Ekstrøm,
Frank Møller Aarestrup,
Sünje Johanna Pamp
Publication year - 2022
Publication title -
microbiology spectrum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.502
H-Index - 51
ISSN - 2165-0497
DOI - 10.1128/spectrum.00090-22
Subject(s) - metagenomics , microbiome , computational biology , biology , library , dna sequencing , dna extraction , sample (material) , dna , genetics , polymerase chain reaction , gene , chemistry , chromatography , 16s ribosomal rna
Previous research has reported effects of sample storage conditions and DNA isolation procedures on metagenomics-based microbiome composition; however, the effect of library preparation and DNA sequencing in metagenomics has not been thoroughly assessed. Here, we provide evidence that library preparation and sequencing platform introduce systematic biases in the metagenomic-based characterization of microbial communities.