Open AccessComparison of Methods To Collect Fecal Samples for Microbiome Studies Using Whole-Genome Shotgun Metagenomic Sequencing
Author(s) -
Doratha A Byrd,
Rashmi Sinha,
Kristi L. Hoffman,
Jun Chen,
Xing Hua,
Jianxin Shi,
Nicholas Chia,
Joseph F. Petrosino,
Emily Vogtmann
Publication year - 2020
Publication title -
msphere
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.749
H-Index - 39
ISSN - 2379-5042
DOI - 10.1128/msphere.00827-19
Subject(s) - metagenomics , microbiome , shotgun sequencing , biology , computational biology , feces , shotgun , genome , bioinformatics , genetics , microbiology and biotechnology , gene
A major direction for future microbiome research is implementation of fecal sample collections in large-scale, prospective epidemiologic studies. Studying microbiome-disease associations likely requires microbial data to be pooled from multiple studies. Our findings suggest collection methods that are most optimal to be used standardly across future WGSS microbiome studies.