Open AccessFirst Report of bla VIM-4 - and mcr-9 -Coharboring Enterobacter Species Isolated from a Pediatric Patient
Author(s) -
Kalyan D. Chavda,
Lars F. Westblade,
Michael J. Satlin,
Andrew Hemmert,
Mariana Castanheira,
Stephen G. Jenkins,
Liang Chen,
Barry N. Kreiswirth
Publication year - 2019
Publication title -
msphere
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.749
H-Index - 39
ISSN - 2379-5042
DOI - 10.1128/msphere.00629-19
Subject(s) - colistin , mcr 1 , enterobacter , gene , enterobacteriaceae infections , plasmid , whole genome sequencing , biology , microbiology and biotechnology , sequence (biology) , genome , genetics , enterobacteriaceae , escherichia coli , antibiotics
An Enterobacter hormaechei isolate harboring bla VIM-4 and mcr-9 was recovered from a pediatric patient in a U.S. hospital. The bla VIM-4 and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The mcr-9 gene is located between two insertion elements, IS 903 and IS 1 , but lacks the downstream regulatory genes ( qseC and qseB ) found in other isolates that harbor mcr-9 IMPORTANCE We describe the complete genome assembly and sequence of a clinical Enterobacter isolate harboring both bla VIM-4 and mcr-9 recovered from a pediatric patient in the United States with a history of travel to Egypt. Moreover, to the best of our knowledge, this is the first report of an Enterobacter isolate harboring both bla VIM-4 and mcr-9 from the United States. The bla VIM-4 and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The mcr-9 gene is located between two insertion elements, IS 903 and IS 1 , but lacks the downstream regulatory genes ( qseC and qseB ) found in other isolates that harbor mcr-9 .