Open AccessMCR-5-Producing Colistin-Resistant Cupriavidus gilardii Strain from Well Water in Batna, Algeria
Author(s) -
Zineb Cherak,
Lotfi Loucif,
Mariem Ben Khedher,
A. Moussi,
Amel Benbouza,
Sophie Alexandra Baron,
JeanMarc Rolain
Publication year - 2021
Publication title -
msphere
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.749
H-Index - 39
ISSN - 2379-5042
DOI - 10.1128/msphere.00575-21
Subject(s) - colistin , efflux , biology , microbiology and biotechnology , mcr 1 , gene , whole genome sequencing , strain (injury) , bacteria , multiple drug resistance , plasmid , genome , genetics , drug resistance , escherichia coli , enterobacteriaceae , antibiotics , anatomy
This paper presents the first description of the mcr-5.1 gene in a colistin-resistant Cupriavidus gilardii isolate from well water that supplies a maternity hospital in Algeria. The whole-genome sequence of this strain showed the presence of putative β-lactamase, aac(3)-IVa , and multidrug efflux pump-encoding genes, which could explain the observed multidrug resistance phenotype. Our findings are of great interest, as we highlight a potential contamination route for the spread of mcr genes. IMPORTANCE Colistin resistance mediated by mcr genes in Gram-negative bacteria has gained significant attention worldwide. This is due to the ability of these genes to be horizontally transferred between different bacterial genera and species. Aquatic environments have been suggested to play an important role in the emergence and spread of this resistance mechanism. Here, we describe the first report of an mcr-5 -positive Cupriavidus gilardii aquatic isolate through its isolation from well water in Algeria. The significance of our study is in shedding the light on an important environmental reservoir of mcr genes.