Open AccessGenome Sequence Comparison of Staphylococcus aureus TX0117 and a Beta-Lactamase-Cured Derivative Shows Increased Cationic Peptide Resistance Accompanying Mutations in relA and mnaA
Author(s) -
Mia Jade Sales,
George Sakoulas,
Richard Szubin,
Bernhard Ø. Palsson,
César A. Arias,
Kavindra V. Singh,
Barbara E. Murray,
Jonathan M. Monk
Publication year - 2020
Publication title -
microbiology resource announcements
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.383
H-Index - 35
ISSN - 2576-098X
DOI - 10.1128/mra.01515-19
Subject(s) - staphylococcus aureus , whole genome sequencing , microbiology and biotechnology , cefazolin , biology , peptide , antimicrobial , plasmid , strain (injury) , genome , bacteria , beta (programming language) , genetics , gene , antibiotics , biochemistry , anatomy , computer science , programming language
Staphylococcus aureus strain TX0117 is a methicillin-susceptible bacterium with type A beta-lactamase exhibiting a high cefazolin inoculum effect. TX0117 was cured of blaZ , yielding TX0117c with increased antimicrobial peptide resistance. The sequencing and genome assembly of TX0117 elucidate six mutations between TX0117 and TX0117c, including relA truncation and mnA_1 substitution.