Open AccessComplete Genome Sequences of the Escherichia coli Donor Strains ST18 and MFD pir
Author(s) -
Simon A. Jackson,
Bridget J. Fellows,
Peter C. Fineran
Publication year - 2020
Publication title -
microbiology resource announcements
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.383
H-Index - 35
ISSN - 2576-098X
DOI - 10.1128/mra.01014-20
Subject(s) - escherichia coli , plasmid , genome , nanopore sequencing , bacteria , biology , genetics , computational biology , gene , microbiology and biotechnology
Escherichia coli ST18 and MFD pir are donors commonly used to transfer oriT RP4 -containing plasmids to diverse bacteria via conjugation. ST18 and MFD pir were constructed via multiple genetic manipulations involving several E. coli strains. Here, we used Illumina and Nanopore sequencing to determine the complete genomes of these widely used strains.