Open AccessComplete Genome Sequence of the Multidrug-Resistant Pseudomonas aeruginosa Endemic Houston-1 Strain, Isolated from a Pediatric Patient with Cystic Fibrosis and Assembled Using Oxford Nanopore and Illumina Sequencing
Author(s) -
Jennifer K. Spinler,
Sabeen Raza,
Jessica K. Runge,
Ruth Ann Luna
Publication year - 2019
Publication title -
microbiology resource announcements
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.383
H-Index - 35
ISSN - 2576-098X
DOI - 10.1128/mra.00903-19
Subject(s) - nanopore sequencing , cystic fibrosis , whole genome sequencing , pseudomonas aeruginosa , lineage (genetic) , biology , genetics , strain (injury) , dna sequencing , sequence (biology) , nanopore , genome , microbiology and biotechnology , gene , bacteria , anatomy , materials science , nanotechnology
Hybrid de novo assembly of Illumina/Nanopore sequence data produced complete circular sequences of the chromosome and a plasmid for the multidrug-resistant Pseudomonas aeruginosa Houston-1 strain. This provides a high-quality representative sequence for a lineage endemic to a pediatric cystic fibrosis care center at Texas Children's Hospital.