Closed Genome Sequence Obtained Using Hybrid Nanopore/Illumina Assembly of a Bacillus anthracis Isolate from an Animal-Skin-Drum-Associated Anthrax Case in the United Kingdom
Author(s) -
Steven T. Pullan,
Rory W. Miles,
Kuiama Lewandowski,
Richard Vipond
Publication year - 2018
Publication title -
microbiology resource announcements
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.383
H-Index - 35
ISSN - 2576-098X
DOI - 10.1128/mra.00802-18
Subject(s) - bacillus anthracis , anthrax vaccines , nanopore sequencing , biology , whole genome sequencing , virulence , genetics , genome , anthrax toxin , lineage (genetic) , plasmid , sequence assembly , nanopore , sequence (biology) , gene , computational biology , bacteria , fusion protein , recombinant dna , dna vaccination , gene expression , transcriptome , materials science , nanotechnology
Hybrid de novo assembly of Illumina/Nanopore reads produced a complete closed genome sequence of the chromosome and two virulence plasmids of a Bacillus anthracis isolate from a fatal anthrax case in the United Kingdom linked to imported animal skins/drums; this provides a high-quality representative sequence for this lineage.
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