Open AccessSequencing and Annotation of Duggie and Hocus, Two Subcluster B1 Mycobacteriophages
Author(s) -
Erin L. Doyle,
Alexis N. Burke,
Samuel J. Coy,
Haley R. Miller,
Lilly M. Shatford-Adams,
Reagan M. Petersen,
Kade B. Wehrs,
Dane M. Bowder
Publication year - 2020
Publication title -
microbiology resource announcements
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.383
H-Index - 35
ISSN - 2576-098X
DOI - 10.1128/mra.00432-20
Subject(s) - siphoviridae , mycobacterium smegmatis , biology , lytic cycle , genome , genetics , whole genome sequencing , annotation , gene , computational biology , bacteriophage , mycobacterium tuberculosis , escherichia coli , medicine , tuberculosis , virus , pathology
Two mycobacteriophage genomes were newly sequenced and annotated. Duggie and Hocus were discovered, enriched, and isolated from soil using Mycobacterium smegmatis mc 2 155. The bacteriophages are lytic Siphoviridae and belong to the B1 subcluster. The Hocus and Duggie genomes are highly similar to one another in both nucleotide sequence and gene content.