A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes | Zendy

Hend Altaib | Zendy; Yuka Ozaki | Zendy; Tomoya Kozakai | Zendy; Yassien Badr | Zendy; Izumi Nomura | Zendy; Tohru Suzuki | Zendy

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A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes

Author(s) -

Hend Altaib,

Yuka Ozaki,

Tomoya Kozakai,

Yassien Badr,

Izumi Nomura,

Tohru Suzuki

Publication year - 2019

Publication title -

microbiology resource announcements

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.383

H-Index - 35

ISSN - 2576-098X

DOI - 10.1128/mra.00323-19

Subject(s) - cloning (programming) , restriction enzyme , escherichia coli , cleavage (geology) , molecular cloning , multiple cloning site , vector (molecular biology) , cloning vector , gene , computational biology , biology , restriction site , enzyme , genetics , microbiology and biotechnology , computer science , biochemistry , gene expression , recombinant dna , paleontology , fracture (geology) , programming language

A series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene cloning.

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