Open AccessAccessible and Validated Processing of SARS-CoV-2 from Wastewater
Author(s) -
Nicole C. Rondeau,
Oliver J. Rose,
Lina A Ariyan,
Brian J. Mailloux,
JJ L. Miranda
Publication year - 2021
Publication title -
microbiology resource announcements
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.383
H-Index - 35
ISSN - 2576-098X
DOI - 10.1128/mra.00174-21
Subject(s) - covid-19 , coronavirus , wastewater , virology , pipeline (software) , severe acute respiratory syndrome coronavirus , computer science , computational biology , medicine , environmental science , biology , disease , infectious disease (medical specialty) , pathology , environmental engineering , outbreak , programming language
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of coronavirus disease 2019 (COVID-19), is shed into wastewater. Accessible methods are necessary for processing samples in a myriad of contexts. We optimized a protocol for extracting viral RNA for downstream experiments. Our pipeline was validated with SARS-CoV-2 itself as a matrix recovery and quantitative measurement control.