Accessible and Validated Processing of SARS-CoV-2 from Wastewater | Zendy

Nicole C. Rondeau | Zendy; Oliver J. Rose | Zendy; Lina A. Ariyan | Zendy; Brian J. Mailloux | Zendy; JJ L. Miranda | Zendy

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Accessible and Validated Processing of SARS-CoV-2 from Wastewater

Author(s) -

Nicole C. Rondeau,

Oliver J. Rose,

Lina A. Ariyan,

Brian J. Mailloux,

JJ L. Miranda

Publication year - 2021

Publication title -

microbiology resource announcements

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.383

H-Index - 35

ISSN - 2576-098X

DOI - 10.1128/mra.00174-21

Subject(s) - covid-19 , coronavirus , wastewater , virology , pipeline (software) , severe acute respiratory syndrome coronavirus , computer science , computational biology , medicine , environmental science , biology , disease , infectious disease (medical specialty) , pathology , environmental engineering , outbreak , programming language

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of coronavirus disease 2019 (COVID-19), is shed into wastewater. Accessible methods are necessary for processing samples in a myriad of contexts. We optimized a protocol for extracting viral RNA for downstream experiments. Our pipeline was validated with SARS-CoV-2 itself as a matrix recovery and quantitative measurement control.

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