Open AccessWhole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle
Author(s) -
Vinícius Silva Castro,
Eduardo Eustáquio de Souza Figueiredo,
Tim A. McAllister,
Robin King,
Tim Reuter,
Rodrigo Ortega Polo,
Carlos Adam Conte,
Kim Stanford
Publication year - 2020
Publication title -
microbiology resource announcements
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.383
H-Index - 35
ISSN - 2576-098X
DOI - 10.1128/mra.00168-20
Subject(s) - escherichia coli , feces , shiga toxin , biology , genome , feedlot , microbiology and biotechnology , genetics , gene , zoology
Forty-eight Escherichia coli strains were chosen due to variable detection of stx or serogroup by PCR. Although all strains were initially determined to be Shiga toxin-producing Escherichia coli (STEC), their genomes revealed 11 isolates carrying stx 1a , stx 1b , stx 2a , and/or stx 2b . Assembled genome sizes varied between 4,667,418 and 5,556,121 bp, with N 50 values between 79,648 and 294,166 bp and G+C contents between 50.