Open AccessStable DNA replication: interplay between DNA replication, homologous recombination, and transcription
Author(s) -
Tokio Kogoma
Publication year - 1997
Publication title -
microbiology and molecular biology reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.358
H-Index - 247
eISSN - 1098-5557
pISSN - 1092-2172
DOI - 10.1128/mmbr.61.2.212-238.1997
Subject(s) - biology , semiconservative replication , control of chromosome duplication , origin recognition complex , eukaryotic dna replication , replication factor c , homologous recombination , dna replication , pre replication complex , replication protein a , genetics , dna , gene , dna binding protein , transcription factor
Chromosome replication in Escherichia coli is normally initiated at oriC, the origin of chromosome replication. E. coli cells possess at least three additional initiation systems for chromosome replication that are normally repressed but can be activated under certain specific conditions. These are termed the stable DNA replication systems. Inducible stable DNA replication (iSDR), which is activated by SOS induction, is proposed to be initiated from a D-loop, an early intermediate in homologous recombination. Thus, iSDR is a form of recombination-dependent DNA replication (RDR). Analysis of iSDR and RDR has led to the proposal that homologous recombination and double-strand break repair involve extensive semiconservative DNA replication. RDR is proposed to play crucial roles in homologous recombination, double-strand break repair, restoration of collapsed replication forks, and adaptive mutation. Constitutive stable DNA replication (cSDR) is activated in mhA mutants deficient in RNase HI or in recG mutants deficient in RecG helicase. cSDR is proposed to be initiated from an R-loop that can be formed by the invasion of duplex DNA by an RNA transcript, which most probably is catalyzed by RecA protein. The third form of SDR is nSDR, which can be transiently activated in wild-type cells when rapidly growing cells enter the stationary phase. This article describes the characteristics of these alternative DNA replication forms and reviews evidence that has led to the formulation of the proposed models for SDR initiation mechanisms. The possible interplay between DNA replication, homologous recombination, DNA repair, and transcription is explored.