Open AccessComplementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer.
Author(s) -
T. Michael Underhill,
Wayne F. Flintoff
Publication year - 1989
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.9.4.1754
Subject(s) - chinese hamster ovary cell , biology , complementation , transfection , microbiology and biotechnology , methotrexate , dna , gene , hamster , cell culture , genetics , phenotype , immunology
A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.