Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer. | Zendy

T. Michael Underhill | Zendy; Wayne F. Flintoff | Zendy

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Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer.

Author(s) -

T. Michael Underhill,

Wayne F. Flintoff

Publication year - 1989

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.9.4.1754

Subject(s) - chinese hamster ovary cell , biology , complementation , transfection , microbiology and biotechnology , methotrexate , dna , gene , hamster , cell culture , genetics , phenotype , immunology

A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.

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