Amplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells. | Zendy

Tsu-Rong Chiang | Zendy; Lisa McConlogue | Zendy

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Amplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells.

Author(s) -

Tsu-Rong Chiang,

Lisa McConlogue

Publication year - 1988

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.8.2.764

Subject(s) - chinese hamster ovary cell , biology , ornithine decarboxylase antizyme , ornithine decarboxylase , dihydrofolate reductase , microbiology and biotechnology , chinese hamster , heterologous , hamster , vector (molecular biology) , ornithine carbamoyltransferase , expression vector , cell culture , ornithine , enzyme , recombinant dna , biochemistry , genetics , amino acid , gene , arginine

We have developed an amplifiable mammalian expression vector based on the enzyme ornithine decarboxylase (ODC). We show greater than 700-fold amplification of this vector in ODC-deficient Chinese hamster ovary cells. A passive coamplified marker, dihydrofolate reductase (dhfr), was amplified and overexpressed 1,000-fold. This ODC vector was a dominant marker in a variety of cell types and displayed at least 300-fold amplification in wild-type Chinese hamster ovary cells.

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