Open AccessAmplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells.
Author(s) -
Tsu-Rong Chiang,
Lisa McConlogue
Publication year - 1988
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.8.2.764
Subject(s) - chinese hamster ovary cell , biology , ornithine decarboxylase antizyme , ornithine decarboxylase , dihydrofolate reductase , microbiology and biotechnology , chinese hamster , heterologous , hamster , vector (molecular biology) , ornithine carbamoyltransferase , expression vector , cell culture , ornithine , enzyme , recombinant dna , biochemistry , genetics , amino acid , gene , arginine
We have developed an amplifiable mammalian expression vector based on the enzyme ornithine decarboxylase (ODC). We show greater than 700-fold amplification of this vector in ODC-deficient Chinese hamster ovary cells. A passive coamplified marker, dihydrofolate reductase (dhfr), was amplified and overexpressed 1,000-fold. This ODC vector was a dominant marker in a variety of cell types and displayed at least 300-fold amplification in wild-type Chinese hamster ovary cells.