Open AccessExpression and processing of a recombinant human macrophage colony-stimulating factor in mouse cells.
Author(s) -
M. Michele Manos
Publication year - 1988
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.8.11.5035
Subject(s) - biology , glycoprotein , recombinant dna , macrophage colony stimulating factor , complementary dna , microbiology and biotechnology , macrophage , colony stimulating factor , oligosaccharide , kilodalton , glycosylation , biochemistry , gene , in vitro , stem cell , haematopoiesis
A human macrophage colony-stimulating factor encoded by a 4-kilobase cDNA was expressed with bovine papillomavirus vectors in mouse cells. Pulse-chase analyses revealed that the 62-kilodalton (kDa) translation product was glycosylated, cleaved, and efficiently secreted within 1 h of synthesis. The secreted product contained both N-linked and O-linked oligosaccharide chains. Macrophage colony-stimulating factor was present extracellularly as an 80-kDa homodimer and as a multimeric species of greater than 200 kDa that may be associated with other glycoproteins.