Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells. | Zendy

Douglas E. Brash | Zendy; Roger R. Reddel | Zendy; Myra Quanrud | Zendy; Kyu Hwan Yang | Zendy; Michael Farrell | Zendy; Curtis C. Harris | Zendy

Open Access

Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells.

Author(s) -

Douglas E. Brash,

Roger R. Reddel,

Myra Quanrud,

Kyu Hwan Yang,

Michael Farrell,

Curtis C. Harris

Publication year - 1987

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.7.5.2031

Subject(s) - biology , transfection , microbiology and biotechnology , antigen , sv40 large t antigen , cell culture , virus , reporter gene , transformation (genetics) , gene , gene expression , virology , biochemistry , immunology , genetics

Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research