Open AccessStrontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells.
Author(s) -
Douglas E. Brash,
Roger R. Reddel,
M. Quanrud,
Yang Ke,
Michael Farrell,
Curtis C. Harris
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.5.2031
Subject(s) - biology , transfection , microbiology and biotechnology , antigen , sv40 large t antigen , cell culture , virus , reporter gene , transformation (genetics) , gene , gene expression , virology , biochemistry , immunology , genetics
Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).