Open AccessNeutralizing monoclonal antibody against ras oncogene product p21 which impairs guanine nucleotide exchange.
Author(s) -
Satoshi Hattori,
David J. Clanton,
Takaya Satoh,
Shun Nakamura,
Yoshito Kaziro,
Masao Kawakita,
Thomas Y. Shih
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.5.1999
Subject(s) - nucleotide , biology , monoclonal antibody , guanine nucleotide exchange factor , guanine , microbiology and biotechnology , oncogene , virology , antibody , biochemistry , genetics , gene , cell cycle , signal transduction
The neutralizing monoclonal antibody Y13-259 severely hampers the nucleotide exchange reaction between p21-bound and exogenous guanine nucleotides but does not interfere with the association of GDP to p21. These results suggest that the nucleotide exchange reaction is critical for p21 function. Interestingly, the v-ras p21 has a much faster dissociation rate than the p21 of the c-ras proto-oncogene.