Open AccessExtinction of expression of immunoglobulin genes in myeloma X fibroblast somatic cell hybrids.
Author(s) -
Aviva Greenberg,
Rosalie Ber,
Z Kra-Oz,
Reuven Laskov
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.2.936
Subject(s) - biology , immunoglobulin heavy chain , antibody , immunoglobulin light chain , immunoglobulin gene , microbiology and biotechnology , gene , myeloma protein , genetics
Adherent hybrids between immunoglobulin-producing mouse myeloma cells and fibroblasts do not produce immunoglobulin polypeptide chains. These hybrids retained the actively rearranged immunoglobulin genes of the myeloma parental cells but lacked immunoglobulin heavy- and light-chain RNA transcripts. We conclude that the shutoff of immunoglobulin production in these hybrids occurs at the transcription or early processing level.