Open AccessInduction of c-Ha-ras transcription in rat cells by simian virus 40 large T antigen.
Author(s) -
Kaoru Segawa,
Nobuo Yamaguchi
Publication year - 1987
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.7.1.556
Subject(s) - biology , chloramphenicol acetyltransferase , microbiology and biotechnology , transcription (linguistics) , virology , messenger rna , antigen , virus , plasmid , mouse mammary tumor virus , simian , long terminal repeat , gene expression , gene , promoter , immunology , biochemistry , linguistics , philosophy
Rat 3Y1 cells expressing simian virus 40 large T antigen under the control of the mouse mammary tumor virus long terminal repeat were established. The amount of c-Ha-ras mRNA in those cells was elevated by about 20 times in parallel with large T antigen after exposure to dexamethasone for 48 h. In chloramphenicol acetyltransferase assays with a plasmid containing the c-Ha-ras-1 promoter the increase in c-Ha-ras mRNA was shown to occur at the transcriptional level.