Open AccessAdenovirus E1A Coding Sequences That Enable ras and pmt Oncogenes To Transform Cultured Primary Cells
Author(s) -
Brad Zerler,
Brian W. Moran,
Kazuo Maruyama,
John F. Moomaw,
Terri Grodzicker,
H E Ruley
Publication year - 1986
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.6.3.887-899.1986
Subject(s) - biology , microbiology and biotechnology , amino acid , gene , plasmid , hek 293 cells , oncogene , mutant , coding region , adenoviridae , peptide sequence , recombinant dna , genetics , cell cycle
Plasmids expressing partial adenovirus early region 1A (E1A) coding sequences were tested for activities which facilitate in vitro establishment (immortalization) of primary baby rat kidney cells and which enable the T24 Harvey ras-related oncogene and the polyomavirus middle T antigen (pmt) gene to transform primary baby rat kidney cells. E1A cDNAs expressing the 289- and 243-amino acid proteins expressed both E1A transforming functions. Mutant hrA, which encodes a 140-amino acid protein derived from the amino-terminal domain shared by the 289- and 243-amino acid proteins, enabled ras (but not pmt) to transform and facilitated in vitro establishment to a low, but detectable, extent. These studies suggest that E1A functions which collaborate with ras oncogenes and those which facilitate establishment are linked. Furthermore, E1A transforming functions are not associated with activities of the 289-amino acid E1A proteins required for efficient transcriptional activation of viral early region promoters.