Open AccessExpression of the denV gene of coliphage T4 in UV-sensitive rad mutants of Saccharomyces cerevisiae.
Author(s) -
Kristoffer Valerie,
Gerald Fronko,
Earl E. Henderson,
J K de Riel
Publication year - 1986
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.6.10.3559
Subject(s) - biology , saccharomyces cerevisiae , pyrimidine dimer , mutant , plasmid , microbiology and biotechnology , gene , gene product , dna glycosylase , photolyase , dna , dna repair , yeast , gene expression , biochemistry
A plasmid containing the denV gene from bacteriophage T4, under the control of the yeast alcohol dehydrogenase I (ADC1) promoter, conferred a substantial increase in UV resistance in the UV-sensitive Saccharomyces cerevisiae mutants rad1-2 and rad3-2. The UV resistance of the denV+ yeast cells was cell cycle dependent and correlated well with the level of the denV gene product as measured by immunoblotting and by a photoreversal assay for pyrimidine dimer-DNA glycosylase activity.