Expression of the denV gene of coliphage T4 in UV-sensitive rad mutants of Saccharomyces cerevisiae. | Zendy

Kristoffer Valerie | Zendy; Gerald Fronko | Zendy; Earl E. Henderson | Zendy; J. Kim de Riel | Zendy

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Expression of the denV gene of coliphage T4 in UV-sensitive rad mutants of Saccharomyces cerevisiae.

Author(s) -

Kristoffer Valerie,

Gerald Fronko,

Earl E. Henderson,

J. Kim de Riel

Publication year - 1986

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.6.10.3559

Subject(s) - biology , saccharomyces cerevisiae , pyrimidine dimer , mutant , plasmid , microbiology and biotechnology , gene , gene product , dna glycosylase , photolyase , dna , dna repair , yeast , gene expression , biochemistry

A plasmid containing the denV gene from bacteriophage T4, under the control of the yeast alcohol dehydrogenase I (ADC1) promoter, conferred a substantial increase in UV resistance in the UV-sensitive Saccharomyces cerevisiae mutants rad1-2 and rad3-2. The UV resistance of the denV+ yeast cells was cell cycle dependent and correlated well with the level of the denV gene product as measured by immunoblotting and by a photoreversal assay for pyrimidine dimer-DNA glycosylase activity.

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