Growth factor requirements of oncogene-transformed NIH 3T3 and BALB/c 3T3 cells cultured in defined media. | Zendy

Xi Zhan | Zendy; Mitchell Goldfarb | Zendy

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Growth factor requirements of oncogene-transformed NIH 3T3 and BALB/c 3T3 cells cultured in defined media.

Author(s) -

Xi Zhan,

Mitchell Goldfarb

Publication year - 1986

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.6.10.3541

Subject(s) - 3t3 cells , fibroblast growth factor , biology , oncogene , growth factor , platelet derived growth factor receptor , platelet derived growth factor , microbiology and biotechnology , fibroblast , transformation (genetics) , cell culture , cell cycle , cell , biochemistry , transfection , genetics , receptor , gene

We report conditions for the efficient growth of NIH 3T3 and BALB/c 3T3 cells cultured in a defined medium supplemented with either platelet-derived growth factor (PDGF) or pituitary-derived fibroblast growth factor (FGF). The oncogenes v-mos, v-src, v-sis, and c-H-ras Val 12 can induce morphological transformation of these cells and can release them from the mitogen requirement for growth, while the oncogene v-fos cannot abrogate the PDGF-FGF requirement. The radically different behavior of normal and transformed NIH 3T3 cells in PDGF-FGF-free defined medium can form the basis of a sensitive new fibroblast transformation assay.

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