Open AccessGrowth factor requirements of oncogene-transformed NIH 3T3 and BALB/c 3T3 cells cultured in defined media.
Author(s) -
Xi Zhan,
Mitchell Goldfarb
Publication year - 1986
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.6.10.3541
Subject(s) - 3t3 cells , fibroblast growth factor , biology , oncogene , growth factor , platelet derived growth factor receptor , platelet derived growth factor , microbiology and biotechnology , fibroblast , transformation (genetics) , cell culture , cell cycle , cell , biochemistry , transfection , genetics , receptor , gene
We report conditions for the efficient growth of NIH 3T3 and BALB/c 3T3 cells cultured in a defined medium supplemented with either platelet-derived growth factor (PDGF) or pituitary-derived fibroblast growth factor (FGF). The oncogenes v-mos, v-src, v-sis, and c-H-ras Val 12 can induce morphological transformation of these cells and can release them from the mitogen requirement for growth, while the oncogene v-fos cannot abrogate the PDGF-FGF requirement. The radically different behavior of normal and transformed NIH 3T3 cells in PDGF-FGF-free defined medium can form the basis of a sensitive new fibroblast transformation assay.