Open Accessp53 cellular tumor antigen: analysis of mRNA levels in normal adult tissues, embryos, and tumors.
Author(s) -
A. M. Rogel,
Malka Popliker,
Cynthia Webb,
Moshe Oren
Publication year - 1985
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.5.10.2851
Subject(s) - biology , messenger rna , embryo , spleen , antigen , embryonic stem cell , organogenesis , andrology , microbiology and biotechnology , immunology , gene , genetics , medicine
The relative levels of mRNA specific for the mouse p53 cellular tumor antigen were determined in various normal adult tissues, embryos, and tumors. All tumors studied contained concentrations of p53 mRNA well above those present in most normal tissues. Normal spleen, however, had p53 mRNA levels comparable to those found in some tumors, despite the fact that they contained barely detectable p53 protein. This apparent discrepancy was found to be due to the extremely rapid turnover rate of p53 in the spleen (half-life, approximately equal to 6 min). In developing fetuses, a marked reduction of p53 mRNA levels was manifest from day 11 onwards, whereas the levels during organogenesis (days 9 to 11) were comparable to those found in undifferentiated embryonic stem cells and in some tumors.