Open AccessStructure of the SAD mutation and the location of control sites at silent mating type genes in Saccharomyces cerevisiae.
Author(s) -
James Hicks,
Jeffrey N. Strathern,
A Klar,
Sajida Ismail,
James R. Broach
Publication year - 1984
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.4.7.1278
Subject(s) - biology , genetics , mating type , gene , expression cassette , mutation , gene duplication , saccharomyces cerevisiae , phenotype , vector (molecular biology) , recombinant dna
The SAD mutation, an extra mating type cassette, has been shown to arise from an unequal mitotic crossover between the MAT and HMR loci, resulting in the formation of a hybrid cassette and a duplication of the MAT-HMR interval. The SAD cassette contains the "a" information and left-hand flanking regions from the parental HMRa cassette and the right-hand flanking sequences of the parental MAT cassette. This arrangement of flanking sequences causes a leaky but reproducible mating phenotype correlated with a low-level expression of the cassette as measured by RNA blotting. This weak expression is attributed to the loss of one flanking control site normally present at the silent HM storage loci.