Open AccessRNA polymerase activity in virions from Ustilago maydis.
Author(s) -
Bat-Sheva Ben-Tzvi,
Y. Koltin,
Moshe Mevarech,
Aviva Tamarkin
Publication year - 1984
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.4.1.188
Subject(s) - rna , biology , rna dependent rna polymerase , small nuclear rna , polymerase , ustilago , microbiology and biotechnology , rna editing , rna polymerase i , rna polymerase , dna , biochemistry , gene
RNA polymerase activity is associated with the double-stranded RNA virions of Ustilago maydis. The reaction products of the polymerase activity are single-stranded RNA molecules. The RNA molecules synthesized are homologous to the three classes of double-stranded RNA molecules that typify the viral genome. The single-stranded RNA synthesized is released from the virions. The molecular weight of the single-stranded RNA transcripts is about half the size of the double-stranded RNA segments, and thus, it appears that in the in vitro reaction, full-length transcripts can be obtained.