RNA and homology mapping of two DNA fragments with repressible acid phosphatase genes from Saccharomyces cerevisiae. | Zendy

N Andersen | Zendy; G P Thill | Zendy; R Krämer | Zendy

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RNA and homology mapping of two DNA fragments with repressible acid phosphatase genes from Saccharomyces cerevisiae.

Author(s) -

N Andersen,

G P Thill,

R Krämer

Publication year - 1983

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.3.4.562

Subject(s) - biology , ecori , microbiology and biotechnology , complementary dna , heteroduplex , homology (biology) , saccharomyces cerevisiae , southern blot , restriction map , restriction enzyme , gene , acid phosphatase , dna , rna , genetics , biochemistry , nucleic acid sequence , enzyme

Two EcoRI restriction fragments carrying Saccharomyces cerevisiae repressible acid phosphatase genes were analyzed. Transcripts were mapped by restriction endonuclease cleavage of glyoxal-stabilized R-loops and by gel blot hybridizations to cDNA. Homology between the two fragments was examined by gel blots and heteroduplex analysis. Each fragment carried a region of about 1.5 kilobases that coded for a repressible acid phosphatase, and these regions showed homology to one another. In addition, one fragment carried a second region of somewhat lower homology that probably codes for the so-called constitutive acid phosphatase.

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