Open AccessRNA and homology mapping of two DNA fragments with repressible acid phosphatase genes from Saccharomyces cerevisiae.
Author(s) -
Nancy Andersen,
G P Thill,
Richard A. Kramer
Publication year - 1983
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.3.4.562
Subject(s) - biology , ecori , microbiology and biotechnology , complementary dna , heteroduplex , homology (biology) , saccharomyces cerevisiae , southern blot , restriction map , restriction enzyme , gene , acid phosphatase , dna , rna , genetics , biochemistry , nucleic acid sequence , enzyme
Two EcoRI restriction fragments carrying Saccharomyces cerevisiae repressible acid phosphatase genes were analyzed. Transcripts were mapped by restriction endonuclease cleavage of glyoxal-stabilized R-loops and by gel blot hybridizations to cDNA. Homology between the two fragments was examined by gel blots and heteroduplex analysis. Each fragment carried a region of about 1.5 kilobases that coded for a repressible acid phosphatase, and these regions showed homology to one another. In addition, one fragment carried a second region of somewhat lower homology that probably codes for the so-called constitutive acid phosphatase.