Open AccessDNA-mediated transfer of multiple drug resistance and plasma membrane glycoprotein expression.
Author(s) -
Paul G. Debenham,
Norbert Kartner,
Louis Siminovitch,
John R. Riordan,
Victor Ling
Publication year - 1982
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.2.8.881
Subject(s) - chinese hamster ovary cell , biology , mutant , p glycoprotein , microbiology and biotechnology , glycoprotein , phenotype , dna , gene , colchicine , drug resistance , cell culture , genetics , multiple drug resistance
Colchicine-resistant Chinese hamster ovary (CHO) cell mutants whose resistance results from reduced drug permeability have been isolated previously in our laboratories. This reduced permeability affects a wide range of unrelated drugs, resulting in the mutants displaying a multiple drug resistance phenotype. A 170,000-dalton cell surface glycoprotein (P-glycoprotein) was identified, and its expression appears to correlate with the degree of resistance. In this study we were able to confer the multiple drug resistance phenotype on sensitive mouse L cells by DNA-mediated gene transfer of DNA obtained from the colchicine-resistant mutants. P-glycoprotein was detected in plasma membranes of these DNA transformants by staining with an antiserum raised against membranes of mutant CHO cells. These results are consistent with a causal relationship between P-glycoprotein expression and the multiple drug resistance phenotype.