Open AccessTargeting frequency for deletion vectors in embryonic stem cells.
Author(s) -
Hongbing Zhang,
Paul Hasty,
Allan Bradley
Publication year - 1994
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.14.4.2404
Subject(s) - biology , embryonic stem cell , hypoxanthine guanine phosphoribosyltransferase , homologous recombination , genetics , stem cell , locus (genetics) , gene targeting , microbiology and biotechnology , chimera (genetics) , hypoxanthine phosphoribosyltransferase , gene , mutant
We analyzed the gene targeting frequencies and recombination products generated by a series of replacement deletion vectors which target the hprt (hypoxanthine phosphoribosyltransferase) locus in mouse embryonic stem cells. We found that the targeting frequency of a 19.2-kb deletion was comparable to that of a 3-kb deletion or a conventional replacement event in which a 1.7-kb fragment was inserted into the locus. We also observed different integration patterns for these deletion vectors. A result of this finding is that a wide range of genomic deletions in embryonic stem cells is feasible.