Open AccessMeasurements of Excision Repair Tracts Formed during Meiotic Recombination in Saccharomyces cerevisiae
Author(s) -
Peter J. Detloff,
Thomas D. Petes
Publication year - 1992
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.12.4.1805-1814.1992
Subject(s) - heteroduplex , saccharomyces cerevisiae , biology , homologous recombination , genetics , gene conversion , meiosis , genetic recombination , recombination , gene , mutant , homologous chromosome , dna repair , flp frt recombination , yeast , dna
During meiotic recombination in the yeast Saccharomyces cerevisiae, heteroduplexes are formed at a high frequency between HIS4 genes located on homologous chromosomes. Using mutant alleles of the HIS4 gene that result in poorly repaired mismatches in heteroduplex DNA, we find that heteroduplexes often span a distance of 1.8 kb. In addition, we show that about one-third of the repair tracts initiated at well-repaired mismatches extend 900 bp.