Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein. | Zendy

René Le Guellec | Zendy; J Paris | Zendy; Antoine Couturier | Zendy; C Roghi | Zendy; M. Philippe | Zendy

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Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein.

Author(s) -

René Le Guellec,

J Paris,

Antoine Couturier,

C Roghi,

M. Philippe

Publication year - 1991

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.11.6.3395

Subject(s) - biology , xenopus , kinesin , complementary dna , polysome , microbiology and biotechnology , gene , cdna library , aspergillus nidulans , cloning (programming) , genetics , differential display , microtubule , rna , ribosome , mutant , computer science , programming language

By differential screening of a Xenopus egg cDNA library, we selected nine clones (Eg1 to Eg9) corresponding to mRNAs which are deadenylated and released from polysomes soon after fertilization. The sequence of one of these clones (Eg5) revealed that the corresponding protein has the characteristic features of a kinesin-related protein. More specifically, Eg5 was found to be nearly 30% identical to a kinesin-related protein encoded by bimc, a gene involved in nuclear division in Aspergillus nidulans.

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