A cellular factor binds to the herpes simplex virus type 1 transactivator Vmw65 and is required for Vmw65-dependent protein-DNA complex assembly with Oct-1. | Zendy

Peter Xiao | Zendy; John P. Capone | Zendy

Open Access

A cellular factor binds to the herpes simplex virus type 1 transactivator Vmw65 and is required for Vmw65-dependent protein-DNA complex assembly with Oct-1.

Author(s) -

Peter Xiao,

John P. Capone

Publication year - 1990

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.10.9.4974

Subject(s) - herpes simplex virus , histone octamer , biology , microbiology and biotechnology , transactivation , single stranded binding protein , fusion protein , virology , dna , virus , dna binding protein , transcription factor , gene , biochemistry , recombinant dna , nucleosome , histone

The herpes simplex virus transactivator Vmw65 assembles into a multicomponent protein-DNA complex along with the octamer binding protein Oct-1. Using affinity chromatography on columns conjugated with purified Vmw65 fusion protein expressed in Escherichia coli, we demonstrate that a cellular factor, distinct from Oct-1, binds to Vmw65 in the absence of target DNA and is necessary for Vmw65-mediated complex assembly with Oct-1.

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