Synergy between the NF-E1 erythroid-specific transcription factor and the CACCC factor in the erythroid-specific promoter of the human porphobilinogen deaminase gene. | Zendy

Jon Frampton | Zendy; M Walker | Zendy; Mark Plumb | Zendy; Paul R. Harrison | Zendy

Open Access

Synergy between the NF-E1 erythroid-specific transcription factor and the CACCC factor in the erythroid-specific promoter of the human porphobilinogen deaminase gene.

Author(s) -

Jon Frampton,

M Walker,

Mark Plumb,

Paul R. Harrison

Publication year - 1990

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.10.7.3838

Subject(s) - porphobilinogen deaminase , biology , promoter , microbiology and biotechnology , transcription factor , transcription (linguistics) , gene , genetics , gene expression , enzyme , biochemistry , heme , linguistics , philosophy

A 114-base-pair promoter fragment of the human porphobilinogen deaminase gene functioned in an erythroid-specific manner in transient transfection experiments. Site-directed mutagenesis of the binding site for the erythroid-specific transcription factor (NF-E1) or an adjacent CACCC motif abolished the promoter activity. Increasing the spacing between these sites progressively reduced promoter activity, but there was no evidence that a critical alignment of the two factors on the DNA helix was required.

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