Open AccessTransformation of NIH 3T3 cells by overexpression of the normal coding sequence of the rat neu gene.
Author(s) -
E Di Marco,
J H Pierce,
C L Knicley,
P P Di Fiore
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.6.3247
Subject(s) - 3t3 cells , biology , gene , oncogene , coding region , microbiology and biotechnology , transformation (genetics) , erbb , gene expression , cell culture , cancer research , transfection , genetics , cell cycle
While the normal human erbB-2 gene is potently transforming when overexpressed in NIH 3T3 cells, its rat homolog, the neu gene, seems to acquire transforming properties only upon alteration of its coding sequence. In this study, we compared the effects of different levels of expression of normal erbB-2 and neu in NIH 3T3 cells. Our results revealed that the normal rat neu gene acts as a potent oncogene when sufficiently overexpressed in NIH 3T3 cells.