Open AccessSp1 can displace GHF-1 from its distal binding site and stimulate transcription from the growth hormone gene promoter.
Author(s) -
Frédéric P. Lemaigre,
D A Lafontaine,
Stéphane Courtois,
S M Durviaux,
Guy Rousseau
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.4.1811
Subject(s) - biology , transcription (linguistics) , cooperativity , dna footprinting , binding site , footprinting , sp1 transcription factor , transcription factor , promoter , microbiology and biotechnology , dna binding protein , cooperative binding , gene , gene expression , genetics , philosophy , linguistics
DNase I footprinting experiments showed that binding activities of Sp1 and of GHF-1 to its distal site on the human growth hormone gene promoter are mutually exclusive. The kinetics of GHF-1 binding were indicative of positive cooperativity. The Sp1 site did not affect promoter activity in cell-free transcription. Still, Sp1 could compensate partially for the decreased stimulation of transcription seen at low GHF-1 concentrations.