Sp1 can displace GHF-1 from its distal binding site and stimulate transcription from the growth hormone gene promoter. | Zendy

Frédéric P. Lemaigre | Zendy; Dominique A. Lafontaine | Zendy; Stéphane Courtois | Zendy; S M Durviaux | Zendy; Guy Rousseau | Zendy

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Sp1 can displace GHF-1 from its distal binding site and stimulate transcription from the growth hormone gene promoter.

Author(s) -

Frédéric P. Lemaigre,

Dominique A. Lafontaine,

Stéphane Courtois,

S M Durviaux,

Guy Rousseau

Publication year - 1990

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.10.4.1811

Subject(s) - biology , transcription (linguistics) , cooperativity , dna footprinting , binding site , footprinting , sp1 transcription factor , transcription factor , promoter , microbiology and biotechnology , dna binding protein , cooperative binding , gene , gene expression , genetics , philosophy , linguistics

DNase I footprinting experiments showed that binding activities of Sp1 and of GHF-1 to its distal site on the human growth hormone gene promoter are mutually exclusive. The kinetics of GHF-1 binding were indicative of positive cooperativity. The Sp1 site did not affect promoter activity in cell-free transcription. Still, Sp1 could compensate partially for the decreased stimulation of transcription seen at low GHF-1 concentrations.

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