The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing. | Zendy

Manley Huang | Zendy; C Gorman | Zendy

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The simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing.

Author(s) -

Manley Huang,

C Gorman

Publication year - 1990

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.10.4.1805

Subject(s) - biology , intron , rna splicing , chloramphenicol acetyltransferase , coding region , genetics , complementary dna , splice site mutation , splice , microbiology and biotechnology , gene , rna , gene expression , reporter gene

Polymerase chain reaction analysis was used to identify aberrant splicing of the simian virus 40 small-t intron present in pRSVcat. We examined factors governing the selection and relative use of aberrant 5' splice sites derived from the chloramphenicol acetyltransferase-coding region. Our results indicated that transcripts from virtually any cDNA positioned upstream of the small-t intron could contain alternative 5' splice sites and therefore be subject to deletions within the protein-coding region.

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