Open AccessThe simian virus 40 small-t intron, present in many common expression vectors, leads to aberrant splicing.
Author(s) -
Manley Huang,
Cornelia M. Gorman
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.4.1805
Subject(s) - biology , intron , rna splicing , chloramphenicol acetyltransferase , coding region , genetics , complementary dna , splice site mutation , splice , microbiology and biotechnology , gene , rna , gene expression , reporter gene
Polymerase chain reaction analysis was used to identify aberrant splicing of the simian virus 40 small-t intron present in pRSVcat. We examined factors governing the selection and relative use of aberrant 5' splice sites derived from the chloramphenicol acetyltransferase-coding region. Our results indicated that transcripts from virtually any cDNA positioned upstream of the small-t intron could contain alternative 5' splice sites and therefore be subject to deletions within the protein-coding region.