Phosphorylation of the ETS-2 protein: regulation by the T-cell antigen receptor-CD3 complex. | Zendy

Shigeyoshi Fujiwara | Zendy; Shigeki Koizumi | Zendy; R.J. Fisher | Zendy; N K Bhat | Zendy; Takis S. Papas | Zendy

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Phosphorylation of the ETS-2 protein: regulation by the T-cell antigen receptor-CD3 complex.

Author(s) -

Shigeyoshi Fujiwara,

Shigeki Koizumi,

R.J. Fisher,

N K Bhat,

Takis S. Papas

Publication year - 1990

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.10.3.1249

Subject(s) - biology , phosphorylation , jurkat cells , ionomycin , microbiology and biotechnology , concanavalin a , protein phosphorylation , t cell receptor , cd3 , polyacrylamide gel electrophoresis , receptor , gel electrophoresis , antigen , biochemistry , t cell , protein kinase a , immunology , immune system , intracellular , in vitro , cd8 , enzyme

Phosphorylation of the human ets-2 protein in response to mitogenic signals to T lymphocytes was investigated in Jurkat cells. Activation of the cells by antibodies against the T-cell antigen receptor-CD3 complex or by concanavalin A was followed within 5 min by increased phosphorylation of the protein, as shown by a mobility shift of the protein from 54 to 56 kilodaltons in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and increased incorporation of 32P. The Ca2+ ionophores A23187 and ionomycin were able to mimic this effect, suggesting that this phosphorylation is mediated by Ca2+.

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