Open AccessPhosphorylation of the ETS-2 protein: regulation by the T-cell antigen receptor-CD3 complex.
Author(s) -
Shigeyoshi Fujiwara,
Shigeki Koizumi,
Robert J. Fisher,
Narayan K. Bhat,
Takis S. Papas
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.3.1249
Subject(s) - biology , phosphorylation , jurkat cells , ionomycin , microbiology and biotechnology , concanavalin a , protein phosphorylation , t cell receptor , cd3 , polyacrylamide gel electrophoresis , receptor , gel electrophoresis , antigen , biochemistry , t cell , protein kinase a , immunology , immune system , intracellular , in vitro , cd8 , enzyme
Phosphorylation of the human ets-2 protein in response to mitogenic signals to T lymphocytes was investigated in Jurkat cells. Activation of the cells by antibodies against the T-cell antigen receptor-CD3 complex or by concanavalin A was followed within 5 min by increased phosphorylation of the protein, as shown by a mobility shift of the protein from 54 to 56 kilodaltons in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and increased incorporation of 32P. The Ca2+ ionophores A23187 and ionomycin were able to mimic this effect, suggesting that this phosphorylation is mediated by Ca2+.