Open AccessE1a transactivation of human HSP70 gene promoter substitution mutants is independent of the composition of upstream and TATA elements.
Author(s) -
Ian Taylor,
Robert E. Kingston
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.1.176
Subject(s) - biology , tata box , promoter , transactivation , upstream activating sequence , transcription factor , transcription (linguistics) , response element , gene , microbiology and biotechnology , mutant , hsf1 , genetics , hsp70 , gene expression , heat shock protein , linguistics , philosophy
We have analyzed 41 deletion, linker scan, and substitution mutants of the human HSP70 gene promoter for activation by the adenovirus E1a region. No natural element of the HSP70 gene promoter was required for activation. To investigate specific interactions between E1a and transcription factors, a set of 24 promoters containing all possible combinations of eight different upstream or TATA motifs was investigated for E1a stimulation. E1a transactivated the promoter regardless of the particular TATA motif present. Furthermore, there was no dramatic correlation between any upstream motif and activation by E1a. These data suggest that E1a does not stimulate transcription via an interaction with any specific transcription factor but instead suggest that E1a interacts via the general transcription machinery.