hnRNP K Supports High-Amplitude D Site-Binding Protein mRNA (Dbp mRNA) Oscillation To Sustain Circadian Rhythms | Zendy

Paul Kwangho Kwon | Zendy; KyungHa Lee | Zendy; Ji-hyung Kim | Zendy; Sookil Tae | Zendy; Seokjin Ham | Zendy; YoungHun Jeong | Zendy; Sung Wook Kim | Zendy; Byunghee Kang | Zendy; HyoMin Kim | Zendy; JungHyun Choi | Zendy; Hee Yi | Zendy; HyunOk Ku | Zendy; TaeYoung Roh | Zendy; Chunghun Lim | Zendy; KyongTai Kim | Zendy

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hnRNP K Supports High-Amplitude D Site-Binding Protein mRNA (Dbp mRNA) Oscillation To Sustain Circadian Rhythms

Author(s) -

Paul Kwangho Kwon,

KyungHa Lee,

Ji-hyung Kim,

Sookil Tae,

Seokjin Ham,

YoungHun Jeong,

Sung Wook Kim,

Byunghee Kang,

HyoMin Kim,

JungHyun Choi,

Hee Yi,

HyunOk Ku,

TaeYoung Roh,

Chunghun Lim,

KyongTai Kim

Publication year - 2020

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.00537-19

Subject(s) - biology , gene knockdown , messenger rna , microbiology and biotechnology , chromatin immunoprecipitation , ribonucleoprotein , gene expression , circadian rhythm , immunoprecipitation , heterogeneous nuclear ribonucleoprotein , transcription (linguistics) , gene , promoter , rna , genetics , endocrinology , linguistics , philosophy

Circadian gene expression is defined by the gene-specific phase and amplitude of daily oscillations in mRNA and protein levels. D site-binding protein mRNA ( Dbp mRNA) shows high-amplitude oscillation; however, the underlying mechanism remains elusive. Here, we demonstrate that heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a key regulator that activates Dbp transcription via the poly(C) motif within its proximal promoter. Biochemical analyses identified hnRNP K as a specific protein that directly associates with the poly(C) motif in vitro Interestingly, we further confirmed the rhythmic binding of endogenous hnRNP K within the Dbp promoter through chromatin immunoprecipitation as well as the cycling expression of hnRNP K. Finally, knockdown of hnRNP K decreased mRNA oscillation in both Dbp and Dbp -dependent clock genes. Taken together, our results show rhythmic protein expression of hnRNP K and provide new insights into its function as a transcriptional amplifier of Dbp .

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