Open AccessViral Manipulation of a Mechanoresponsive Signaling Axis Disassembles Processing Bodies
Author(s) -
Elizabeth L. Castle,
Carolyn-Ann Robinson,
Pauline Douglas,
Kristina D. Rinker,
Jennifer A. Corcoran
Publication year - 2021
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.00399-21
Subject(s) - biology , microbiology and biotechnology , cytoskeleton , rhoa , ectopic expression , effector , actin , ribonucleoprotein , signal transduction , cell , cell culture , rna , genetics , gene
Processing bodies (PBs) are ribonucleoprotein granules important for cytokine mRNA decay that are targeted for disassembly by many viruses. Kaposi’s sarcoma-associated herpesvirus is the etiological agent of the inflammatory endothelial cancer, Kaposi’s sarcoma, and a PB-regulating virus. The virus encodes kaposin B (KapB), which induces actin stress fibers (SFs) and cell spindling as well as PB disassembly. We now show that KapB-mediated PB disassembly requires actin rearrangements, RhoA effectors, and the mechanoresponsive transcription activator, YAP. Moreover, ectopic expression of active YAP or exposure of ECs to mechanical forces caused PB disassembly in the absence of KapB. We propose that the viral protein KapB activates a mechanoresponsive signaling axis and links changes in cell shape and cytoskeletal structures to enhanced inflammatory molecule expression using PB disassembly. Our work implies that cytoskeletal changes in other pathologies may similarly impact the inflammatory environment.