Open AccessAdaptive Evolution of Geobacter sulfurreducens in Coculture with Pseudomonas aeruginosa
Author(s) -
Lucie Semenec,
Ismael A. Vergara,
Andrew Elohim Laloo,
Steve Petrovski,
Philip L. Bond,
Ashley E. Franks
Publication year - 2020
Publication title -
mbio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.562
H-Index - 121
eISSN - 2161-2129
pISSN - 2150-7511
DOI - 10.1128/mbio.02875-19
Subject(s) - geobacter sulfurreducens , geobacter , bacteria , biology , adaptive evolution , pseudomonas aeruginosa , electron transfer , horizontal gene transfer , genome , computational biology , biofilm , microbiology and biotechnology , genetics , chemistry , gene , organic chemistry
Geobacter and Pseudomonas spp. cohabit many of the same environments, where Geobacter spp. often dominate. Both bacteria are capable of extracellular electron transfer (EET) and play important roles in biogeochemical cycling. Although they recently in 2017 were demonstrated to undergo direct interspecies electron transfer (DIET) with one another, the genetic evolution of this syntrophic interaction has not been examined. Here, we use whole-genome sequencing of the cocultures before and after adaptive evolution to determine whether genetic selection is occurring. We also probe their interaction on a temporal level and determine whether their interaction dynamics change over the course of adaptive evolution. This study brings to light the multifaceted nature of interactions between just two microorganisms within a controlled environment and will aid in improving metabolic models of microbial communities comprising these two bacteria.