Open AccessAllosteric Activation of SARS-CoV-2 RNA-Dependent RNA Polymerase by Remdesivir Triphosphate and Other Phosphorylated Nucleotides
Author(s) -
Bing Wang,
Vladimir Svetlov,
Yuri I. Wolf,
Eugene V. Koonin,
Evgeny Nudler,
Irina Artsimovitch
Publication year - 2021
Publication title -
mbio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.562
H-Index - 121
eISSN - 2161-2129
pISSN - 2150-7511
DOI - 10.1128/mbio.01423-21
Subject(s) - rna polymerase , allosteric regulation , rna dependent rna polymerase , nucleoside triphosphate , polymerase , rna , biochemistry , biology , nucleotide , ribosome , enzyme , nucleoside , chemistry , gene
In vitro interrogations of the central replicative complex of SARS-CoV-2, RNA-dependent RNA polymerase (RdRp), by structural, biochemical, and biophysical methods yielded an unprecedented windfall of information that, in turn, instructs drug development and administration, genomic surveillance, and other aspects of the evolving pandemic response. They also illuminated the vast disparity in the methods used to produce RdRp for experimental work and the hidden impact that this has on enzyme activity and research outcomes.