Open AccessLoss of the Bacterial Flagellar Motor Switch Complex upon Cell Lysis
Author(s) -
Mohammed Kaplan,
Elitza I. Tocheva,
Ariane Briegel,
Megan J. Dobro,
YiWei Chang,
Poorna Subramanian,
Alasdair W. McDowall,
Morgan Beeby,
Grant J. Jensen
Publication year - 2021
Publication title -
mbio
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.562
H-Index - 121
eISSN - 2161-2129
pISSN - 2150-7511
DOI - 10.1128/mbio.00298-21
Subject(s) - flagellum , cytoplasm , lysis , microbiology and biotechnology , biology , molecular motor , motor protein , rest (music) , bacteria , microtubule , genetics , biochemistry , physics , acoustics
The bacterial flagellar motor is a complex macromolecular machine whose function and self-assembly present a fascinating puzzle for structural biologists. Here, we report that in diverse bacterial species, cell lysis leads to loss of the cytoplasmic switch complex and associated ATPase before other components of the motor. This loss may be prevented by the formation of a cytoplasmic vesicle around the complex. These observations suggest a relatively loose association of the switch complex with the rest of the flagellar machinery.