Open AccessInteraction of Cellular Proteins with the 5′ End of Norwalk Virus Genomic RNA
Author(s) -
Ana Lorena Gutiérrez-Escolano,
Zamirath Uribe Brito,
Rosa M. del Ángel,
Xi Jiang
Publication year - 2000
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.74.18.8558-8562.2000
Subject(s) - biology , rna , internal ribosome entry site , translation (biology) , viral replication , microbiology and biotechnology , norwalk virus , rna binding protein , virology , poliovirus , gene expression , ribosome , virus , gene , messenger rna , genetics , norovirus
The lack of a susceptible cell line and an animal model for Norwalk virus (NV) infection has prompted the development of alternative strategies to generate in vitro RNAs that approximate the authentic viral genome. This approach has allowed the study of viral RNA replication and gene expression. In this study, using mobility shift and cross-linking assays, we detected several cellular proteins from HeLa and CaCo-2 cell extracts that bind to, and form stable complexes with, the first 110 nucleotides of the 5′ end of NV genomic RNA, a region previously predicted to form a double stem-loop structure. These proteins had molecular weights similar to those of the HeLa cellular proteins that bind to the internal ribosomal entry site of poliovirus RNA. HeLa proteins La, PCBP-2, and PTB, which are important for poliovirus translation, and hnRNP L, which is possibly implicated in hepatitis C virus translation, interact with NV RNA. These protein-RNA interactions are likely to play a role in NV translation and/or replication.