Transformation-defective mutant of adenovirus type 5 containing a single altered E1a mRNA species | Zendy

L R Carlock | Zendy; N C Jones | Zendy

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Transformation-defective mutant of adenovirus type 5 containing a single altered E1a mRNA species

Author(s) -

L R Carlock,

N C Jones

Publication year - 1981

Publication title -

journal of virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.617

H-Index - 292

eISSN - 1070-6321

pISSN - 0022-538X

DOI - 10.1128/jvi.40.3.657-664.1981

Subject(s) - biology , mutant , microbiology and biotechnology , adenoviridae , messenger rna , insert (composites) , rna splicing , transformation (genetics) , rna , virology , hela , viral replication , virus , genetics , cell culture , gene , genetic enhancement , mechanical engineering , engineering

A mutant of adenovirus type 5 containing an octanucleotide insert in region E1a of the viral genome was constructed. The insert was present in only one (13s) of the three overlapping mRNA's synthesized from this region. The insert was within the sequences removed by RNA splicing during the production of the other two nRNA's. The insertion resulted in a shift in the translational reading frame of the 13s mRNA and the probable premature termination of translation. The mutant was defective for viral DNA replication in HeLa cells and the transformation of rat embryo and baby rat kidney cells, indicating that a product encoded by the 13s nRNA is required for these two processes. Other early regions of the genome were expressed in HeLa cells infected by this mutant although in some cases the expression was decreased as compared with wild-type-infected cells.

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