Open AccessPlasma-Derived HIV-1 Virions Contain Considerable Levels of Defective Genomes
Author(s) -
Katie Fisher,
Xiao Qian Wang,
Ashley Lee,
Vincent Morcilla,
Anneke de Vries,
Eunok Lee,
JohnSebastian Eden,
Steven G. Deeks,
Anthony D. Kelleher,
Sarah Palmer
Publication year - 2022
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.02011-21
Subject(s) - genome , biology , rna , virology , dna , peripheral blood mononuclear cell , deep sequencing , dna sequencing , virus , integrase , lentivirus , genetics , human immunodeficiency virus (hiv) , microbiology and biotechnology , computational biology , gene , viral disease , in vitro
We developed novel plasma-derived RNA using long-range sequencing assays (PRLS assay; 8.3 kb,gag -3′, and 5.0 kb,int -3′). Employing thegag -3′ PRLS assay, we found that 26% to 51% of plasma-derived genomes are genetically defective, largely as a result of frameshift mutations and deletions.