Open AccessDevelopment of a Single-Cycle Infectious SARS-CoV-2 Virus Replicon Particle System for Use in Biosafety Level 2 Laboratories
Author(s) -
Johnny R. Malicoat,
Senthamizharasi Manivasagam,
Sonia Zúñiga,
Isabel Sola,
Dianne McCabe,
Lijun Rong,
Stanley Perlman,
Luis Enjuanes,
Balaji Manicassamy
Publication year - 2022
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.01837-21
Subject(s) - biology , luciferase , virology , vesicular stomatitis virus , virus , green fluorescent protein , replicon , transfection , viral replication , reporter gene , plasmid , cell culture , gene expression , gene , genetics
Due to the highly contagious nature of SARS-CoV-2 and the lack of immunity in the human population, research on SARS-CoV-2 has been restricted to biosafety level 3 laboratories. This has greatly limited participation of the broader scientific community in SARS-CoV-2 research and thus has hindered the development of vaccines and antiviral drugs.