Open AccessMechanical Capsid Maturation Facilitates the Resolution of Conflicting Requirements for Herpesvirus Assembly
Author(s) -
Alex Evilevitch,
Udom Sae-Ueng
Publication year - 2022
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.01831-21
Subject(s) - capsid , biology , biophysics , microbiology and biotechnology , herpes simplex virus , dna , virology , virus , genetics
The minor capsid protein UL25 plays a critical role in the mechanical maturation of the HSV-1 capsid during virus assembly and is required for stable DNA packaging. We modulated the UL25 capsid interactions by genetically deleting different UL25 regions and quantifying the effect on mechanical capsid stability using an atomic force microscopy (AFM) nanoindentation approach.