Open AccessMechanism through Which Retrocyclin Targets Flavivirus Multiplication
Author(s) -
Xiaoying Jia,
Jiao Guo,
Weirong Yuan,
Lingling Sun,
Yang Liu,
Siqi Dong,
Gengfu Xiao,
Wuyuan Lu,
Alfredo Garzino-Demo,
Wei Wang
Publication year - 2021
Publication title -
journal of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.617
H-Index - 292
eISSN - 1070-6321
pISSN - 0022-538X
DOI - 10.1128/jvi.00560-21
Subject(s) - flavivirus , ns3 , biology , virology , serine protease , protease , viral replication , virus , zika virus , hepatitis c virus , biochemistry , enzyme
Retrocyclin is an artificially humanized circular θ-defensin peptide, containing 18 residues, previously reported to possess broad antimicrobial activity. In this study, we found that retrocyclin-101 inhibited flavivirus (ZIKV and JEV) infections. Retrocyclin-101 inhibited NS2B-NS3 serine protease activity, suggesting that the catalytic triad of the protease is the target. Moreover, retrocyclin-101 bound to the DE loop of the E protein of flavivirus, which prevented its entry.